Cloning and phylogenetic analysis of the NSP1~5 genes of giant panda rotavirus strain CH-1 isolated in China

Abstract

Rotavirus (RV), a member of the genus Rotavirus of the family Reoviridae, is an important causative agent of diarrhoea diseases of human and animals worldwide. The Asian Rotavirus Surveillance Network reported that overall 45% of diarrhoea admissions in Asian region were positive for rotavirus in nine countries and regions of Asia (2008). But very limited knowledge about animal diarrhoea diseases caused by rotavirus was published until now. Valuation of genetic relations between human and animal rotavirus isolates is very limited. To better understand the rotavirus CH-1 strain isolated from diarrheic faeces of giant panda in 2008, we cloned the non-structural protein (NSP) 1~5 complete coding sequence of the giant panda rotavirus (GPRV), and sequenced NSP1~5 genes (GenBank accession number: NSP1, GU205762; NSP2, GU188281; NSP3, GU329525; NSP4, GU188282; NSP5, GU329526). Based on these information and data from GenBank of other genus of RV NSP1~5 genes, phylogenetic analysis were realized. The phylogenetic tree revealed that GPRV NSP1~5 genes were close to that of porcine rotavirus, bovine rotavirus and human rotavirus. This research may provide some useful information helping us understanding giant panda rotavirus.