Antibacterial evaluation and phytochemical screening of Methanolic Extract of Ocimum sanctum against some common microbial pathogens

Abstract

Whole plant methanolic extract of Ocimum sanctum was studied for their phytochemical and
antibacterial activities. Antimicrobial activity of extract was detected against some common microbial
pathogens. Methanolic extract of Ocimum sanctum exhibited antibacterial activity against
Enterococcus faecalis, Enterobacter cloacae, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae,
Staphylococcus aureus and Staphylococcus saprophyticus. Methanolic extract of Ocimum sanctum
showed strong antibacterial activity against Staphylococcus aureus and Staphylococcus
saprophyticus. The minimal inhibitory concentrations for all tested strains were at concentrations
ranging from 10.19±0.08mg/ml to 32.02±1.98 mg/ml. Phytochemical analysis of active extracts
demonstrated the presence of common phytoconstituents like tannins, glycosides, saponins,
flavonoids and alkaloids. These phytochemical established a good support to the use of this plant in
herbal medicine and as a base for the development of new drugs, phytomedicine and might be aid in
the treatment of UTI.