Antagonistic bacteria affecting the Golden cyst potato nematode (Globoderarostochiensis Woll.) in the region of Perote, Veracruz, México

Abstract

The golden cyst nematode (Globoderarostochiensis) presents one of the major problems in potato
farming in highlandregionsin variousparts of the world and México is no exception. The high application
of chemicals to control the nematode and the related environmental problems have led to the search for
alternative control options, such as the use of rhizobacteria, which have indicatednew ways of achieving
an increase in agricultural productivity as well asbeing an ecological and economically feasible means
to minimize harmful practices in agriculture. The goal of this study was to isolate and evaluate "in vitro "
the antagonistic capacity of rhizobacteria affecting cysts and J2 stages of Globoderarostochiensis.
Nematodes were extracted from soil samples from the localities of Perote and Los Altos de Ayahualulco
and identified morphometric and molecularly. Potato roots from undisturbed areas in the highland
region of Perote were collected for bacterial isolation.The bacteria were isolated on media King B, MB
and nutrient agar, and were identified byananalysis ofthe 16S rRNA subunit gene. 102 bacterial isolates
were evaluated on J2 and G. rostochiensis cysts. Ten of the bacterial isolates were seen to cause
distortion and disintegration of J2 nematode individuals. None of the bacterial isolates caused damage
in cysts. The cysts provide the female with a defense strategy, which makes them difficult to control. We
found that promising bacterial species corresponded to the generas Providencia, Pseudomonas,
Paracoccus and Serratia.