Distribution of free-living amoebae in springs in Morelos, Mexico

Abstract

Free-living amoebae (FLA) can accomplish their life cycle as free-living organisms; therefore it is
important to know more about the distribution of FLA in the environment. The aim of the study was to
determine the presence and distribution of free-living amoebae in recreational and drinking water
springs. Eight springs were selected and the samples were taken on a bimonthly basis for one year.
Filtration of water samples was performed and culture was done on non-nutrient agar medium with
Enterobacter aerogenes bacteria. Identification of FLA was carried out taking in account the
morphological characteristics of the trophozoite and cyst. Forty one of 48 (85.4%) samples tested
positive for the presence of free-living amoebae and isolated amoebae belonging to 15 genera. Genera
were grouping into 4 groups in according to spatial-seasonal distribution, having Naegleria and
Vermamoeba the highest frequency and distribution. The study contributed to knowledge of the
diversity of free-living amoebae in springs and provided interesting information about the spatial and
seasonal distribution FLA in these water bodies.