Production and optimization of invertase from pomegranate peel by marine fungal strain aspergillus terreus

Abstract

Agricultural wastes were the low cost raw material for invertase production by fungi. The marine
fungal strain Aspergillusterreus was characterized and phylogenetic analysis by 18S rRNA gene
sequencing then evaluated for invertase production using 40 g/l of pomegranate peel (Pp) as
substrate. Maximum invertase activity was 264.4 U/ml after 5 days incubation period. Two disks
6mm in diameter, pH 6.5 and 30 ◦C were the optimum conditions for invertase production. 3% of
triton x100 as surfactant produce 281.2 U/ml .Increasing invertase production by addition of
wheat bran ,EDTA and cysteine to pomegranate peel.