Fecal carriage of extended-spectrum β-lactamase producing Enterobactearicae: a comparative study between hospitalized and non-hospitalized patients

Abstract

Recently the world has seen a surge in extended-spectrum β-lactamase (ESBL)-producing bacteria.
However, data on the dissemination of ESBL-producing Enterobacteriaceae in the hospital and in non
hospitalized personal from systematically enrolled study subjects in Egypt remains few. Aim of study: To
determine the prevalence, phenotypic resistance patterns and genetic characteristics of ESBL-producing
Entearobacteracicea in fecal carriage in both hospital and in non-hospitalized personal. Material and
methods: A total of 160 fecal samples from 60 non hospitalized coming from out patients clinics and100
hospitalized patients .The were screened for the presence of ESBL producing Enterobactearicae. A
multiplex PCR assay was used to identify blaCTX-M blaSHV, blaOXA, blaTEM genes. Results: Out of 60
clinical isolates from non-hospitalized patients group (1) it was found that the 41 Escherichia coliisolates
produced the different ESBLs types as follow:(58%) produced TEM gene, (95%) produced CTX-M, (54%)
produced TEM combined with CTX-M. Out of 100 clinical isolates from hospitalized patients group (II) it
was founded that the (50) Escherichia coliisolates produced the different ESBLs types as follow: (78%)
produced TEM gene, (96%) produced CTX-M and (74%) produced TEM combined with CTX-M. Conclusion:
The most affected age was 15-30 years; this may be due to the panting of this age group on fast food,
which may be the reason in the transmission and spread of infection by these microbes. blaCTX-M was
the most prevalent ESBL gene followed by blaTEM and finally, blaSHV, while the blaOXA gene was not
detected in any of the isolated bacteria.