Characterization of Pasteurella multocida strains isolated from cattle and buffaloes in Karachi, Pakistan
Keywords:
Pasteurella multocida, phylogenetic analysis, PCR, 16S ribosomal DNA, serotyping.Abstract
Pasteurella multocida is a Gram-negative, non-motile coccobacillus. The organism is of special significance in the
tropical countries including Pakistan where only in Punjab Province, it caused heavy economics losses as Rs.2.17
billion per year. We Isolated and identified the disease causing serotypes in Pakistan by using cultural, morphological
and biochemical tests. Pathogenicity was confirmed by inoculation of the isolates in rabbits. Serotyping of the isolates
was done and our isolates were found to be P. multocida, B: 2 serotype. Polymerase chain reaction (PCR) of 16s
ribosomal deoxyribonucleic acid (rDNA) was done which amplified 1500 bp deoxyribonucleic acid (DNA) fragment. DNA
sequencing and phylogenetic analysis of the gene revealed that our isolates are different from each other and they
belong to two different groups. Whe
