A morphological, physiological and molecular investigation of fischerella sp. MCCS023 (cyanobacteria) from paddy-fields of Iran

Abstract

Fischerella ambigua is an important member of the cyanobacterial community in the rice field. In this study
morphological, taxonomical variation in blue-green algae Fischerella ambigua was determined. In later stage
they are treated with different nitrogen sources (N-free, 1 & 2 mM NH4+ and 5 and 10 mM NO3−). Results
indicated that maximum growth rate belonged to NO3− treatment. Dry weights of Fischerella sp. MCCS023 in Nfree medium and ammonium were in decreasing order of preference; and NH4 + 2 mM was drastically toxic for
this strain and at last molecularly investigated. For Genetic variation, among rice-field, Fischerella isolates were
studied using DNA extracted from single filament-derived cultures. Laboratory studies have shown that it is
possible to use PCR to amplify individual Cyanobacteria filaments. As a result of these studies, Shiraz
University was isolated for direct PCR and sequence analysis. Our results show that two distinct genotypes of
Fischerella are present in the population at all stations and are not present in approximately equal numbers,
they are not distributed uniformly