Streamlining of procedure parameters for the generation of - amylase from Penicillium janthinellum (NCIM 4960) under solid state fermentation

Abstract

The production of extracellular amylase was studied in solid state fermentation by Penicillium
janthinellum (NCIM 4960) using wheat bran as a solid substrate. The physical and chemical parameters
influencing SSF were optimized. The maximum enzyme activity obtained was 300 U/gds under optimum
conditions of pH 5.0, an incubation temperature of 35°C, maltose (1% w/w) as carbon source, (NH4)2SO4
- (0.2% w/w) as nitrogen source, particle size (425 - 500 µ), incubation time (96 h), moisture content
(60%), and surfactant (Tween-80, 0.05% w/w).