Multidrug resistance among bacteria isolated from some foods sold in Restaurants in Abraka, Nigeria

Abstract

Egusi soup and jollof rice samples were collected from various restaurants in Delta State, Nigeria. Isolation
of the bacteria present was done by culturing, using spread plate technique on Nutrient agar, blood agar
and MacConkey agar plates incubated at 37oC for 24 hours. The bacteria isolated were Bacillus cereus,
Klebsiella, pneumoniae, Escherichia coli, Lactobacillus acidophilus, Bacillus subtilis, Micrococcus luteus
and Acinetobacter calcoaceticus. The total colony count ranged from 8.0x103
to 45.6x103
cfu/g.
K.pneumoniae and E.coli had the highest frequency of occurrence. The plasmid analysis revealed that of
the isolates with resistance, the percentage resistance of the bacterial isolates before plasmid curing was
highest (100%) in Cefixime, Augmentin, Ceftriazone and Cefuoxime while resistance to septrin was the
lowest (60%). After plasmid curing, the percentage resistance of the bacterial isolates was highest in
Cefixime and Cefuroxime(100%) while resistance to Septrin was still the lowest (60%). Agarose gel
electrophoresis revealed that some of the K.pneumoniae, E.coli and B. subtilis isolates harboured multidrug resistant plasmids with DNA sizes less than 23130bp while those that did not harbor multidrug
resistant plasmids included Lactobacillus acidophilus, B. subtilis