PREVALENCE AND DISTRIBUTION OF HIGH-RISK HUMAN PAPILLOMA VIRUS IN CERVICAL LESIONS AND ASYMPTOMATIC HEALTHY SUBJECTS OF CENTRAL INDIA

Abstract

 Background: Detection of human papilloma virus (HPV) infection and its genotype distribution are much-needed parameters to judge the risk of cervical cancer among females. However, due to less availability of data on HPV burden in Central part of India makes cervical cancer screening difficult.

Methods: A total of 270 participants had been tested for abnormalities in cytology (Pap smear) and real-time PCR was performed for HPV DNA detection.

Result: The most common cytological finding among HPV positive patients was found to be of negative for intraepithelial lesion or malignancy NILM (93.7%), followed by atypical squamous cells of undetermined significance (ASCUS) (5.2%), low-grade intraepithelial lesion (LSIL) (0.7%) and high-grade intraepithelial lesion (0.35%) cases. HPV DNA was detected in (8.1%) patients. Highest positivity was seen in the age group of 30-39 years followed by age group of 20-29 years. The most common HPV type in our study was HPV 16 (45.4%) followed by HPV 31 infection (27.2%). Other genotypes of HPV found were HPV18 (13.6%), HPV 51 (2;

9%), HPV 58 (2; 9%), and HPV 39 or 68∗ (9%).

Conclusion: It is evident from our study that HPV DNA infection was observed with normal cervical cytology. For early detection both Pap smear and HPV DNA genotype detection should be the choice for preventing cervical cancer in women.