Genetic diversity analysis of Iranian citrus varieties using micro satellite (SSR) based markers

Abstract

Fifteen SSR Primer Pairs were used to estimate the level of polymorphism among 23 Citrus genotypes and four
natural hybrids or bud mutation was selected from Kotra Germplasm Bank (IRAN) . All fifteen loci assayed in citrus
plant possessed a high level of polymorphism, with the number of alleles per locus ranging from 4 in TAA41 to 12 at
CAT01, ATC09, AG14 ( an average, 8.27 alleles were detected per locus ). Cluster analysis with SSR markers resulted
in 2 cluster groups: Group A: Yuzo and Poncirus. Group B: There are three separate subgroups within Group B; (i)
genus Fortunella sp (ii) Mandarin subgroup: Citrus reticulate (Citrus clemantin), Citrus sinensis (Pineapple,
Washington Navel), Natural types (Siahvaraz, Shalmahaleh, Moallemkoh and Kotra 4 hybrids) and (iii) Citrus Limon
(Amol lemon - pear, Eureka, Rough Lemon), Citrus aurantifolia, Citrus aurantium, Citrus medica and Citrus grandis.
Microsatellite analysis clustered citron and sour orange cv cluster but these taxa were quiet distant from Fortunella
SP.