In a Microbiological Laboratory, Contamination

Abstract

In the biological laboratory, contamination is a well-known issue with grave repercussions. Physical, chemical, and biological are its three primary categories. Bacteria, molds, yeasts, viruses, mycoplasma, and cross-contamination by different cell lines are the most frequent biological contaminants that are encountered. An overview of the main important sources and available controls for pollutants is given in this article. The primary cause of contamination is the unintentional or intentional introduction of pollutants into the intended system from the start of laboratory activity until its conclusion. Obtaining pure and viable cells from reliable gene banks, occasionally assessing the culture's properties, employing antibiotics on a regular basis, and adopting good aseptic technique are just a few of the many tactics that must be used every day to tackle the difficulty. This research generally proposed that visual evaluation of the culture within a few days of infection could minimize or eliminate the frequency and severity of contamination.