Phylogenetic relationship among Mango (Mangifera indica L.) Landraces of Saurashtra based on DNA fingerprinting

Abstract

Twenty mango cultivars (Mangifera indica Linn) collected from Gir region of Saurashtra were examined by ISSR
markers. Of the 50 primers screened, 21 primers gave reproducible, polymorphic DNA amplification patterns, and
were selected to construct a DNA fingerprinting map to distinguish the genotypes of mango. According to the
banding patterns obtained with 21 selected primers, all cultivars tested in this study except Jamadar and Kesar were
distinguished from each other and showed ample genetic diversity, indicating that ISSR-PCR was an effective
method for cultivar identification of mangoes. Based on 125 selected bands, all Gir mango landraces tested were
clustered into a three big groups with ‛Kaju’ and ‛Khodi’ in first group, Dudh Pendo, Sopari, Jamadar, Kesar and
Ashadhiya in second group, while the third cluster was composed of Agargato, Amir Pasand, Pethal, Gajariyo,
Chhappaniyo, Alphanso, Neelum, Jamrukhiyo, Kavasji Patel, Giriraj, Amrutiyo, Dasheri and Deshi based on UPGMA
analysis, indicating that some Gir landraces had a close relationship with each other, while some were drastically
dissimilar from other landraces.