Comparative proteomic analysis of the effects of dietary mugwort (Artemisia iwayomogi Kitamura) on the pig Longissimus dorsi muscle
Keywords:
2-DE, longissimus dorsi muscle, meat quality, mugwort, pig, proteome.Abstract
Proteomic analysis by 2-dimensional electrophoresis (2-DE) of the longissimus dorsi muscle in finishing pigs (LY × D)
grown on a diet supplemented with mugwort powder found roughly 300 spots on a polyacrylamide gel image. Among
them, the expression levels of 12 spots were higher than those of the control group. From the results of peptide mass
fingerprinting (PMF) analysis, nine proteins were characterized: myosin light chain 2V, elF-5A, myosin light chain 1, F1-
ATPase chain D, peroxiredoxin-2, Kelch-related protein 1, SLA-7, glycine amidinotransferase, and Tpi 1. The other three
spots with increased expression were identified by chemically assisted fragmentation-matrix-assisted laser
desorption/ionization (CAF-MALDI) sequencing as serum albumin precursor and two different types of myoglobin.
Taken together, these results indicate that mugwort powder has the potential to improve the quality of pork meat.
