An efficient secretion of the protein fused to the AgfA signal sequence in Salmonella
Keywords:
–Lactamase, thin aggregative fimbriae A, outer membrane protein W, Salmonella typhimurium fimbriae AAbstract
Signal sequence (SS) of surface or secreting proteins plays an important function for protein secretion in bacterial
system. The SS of various proteins may mediate different level of protein excretion yield of the proteins. In order to
examine the effect of SS types in protein secretion, signal sequences of Bla (– lactamase), AgfA (thin aggregative
fimbriae A), StfA (Salmonella typhimurium fimbriae A) and OmpW (outer membrane protein W) were selected for the
secretion of PspA protein which was used as a test protein. The PCR-amplified DNAs corresponding to each SS were
cloned into the plasmid pYA3342. A primer used in PCR was designed to insert a His6-tag at the C- terminal of SS for
the convenient detection of expressed SS. The 0.8 kb EcoRI-Hin dIII pspA gene was cloned into the recombinant
plasmids, resulting pMMP66, pMMP67, pMMP68 and pMMP70. The S. typhimurium strains harboring the recombinant
plasmids expressed the His6-tagged PspA, demonstrating in-frame fusion of PspA to each Depending upon the type of
SS, cell lysate as well as secreted PspA of each Salmonella samples was found different. Relatively, S. typhimurium
containing pMMP67 (carrying AgfA SS) secreted the highest level of PspA than others, and suggested that the AgfA
SS mediates efficient translocation of the PspA. Conclusively, the AgfA SS mediated secretion system in pMMP67 can
be used in variety fields required for the high level of protein secretion, especially antigen delivery in recombinant
attenuated Salmonella vaccines.