Isolation and purification of membrane-bound cytochrome c from Proteus mirabilis

Abstract

In the present studies, respiratory chain pathogenic bacterium, Proteus mirabilis, was investigated. In
the first phase, growth profile study was performed to optimize the P. mirabilis growth. Maximum
bacterial growth could be obtained between 10 – 12 h of culturing time. Down-stream processing was
performed by using sonication, ultracentrifugation and detergent solubilization techniques. Partially
purified respiratory contents were analyzed spectrophotometrically. Pyridine-ferrochrome and redox
spectra showed the presence of heme-c.