An innovative approach for hyperproduction of cellulolytic and hemicellulolytic enzymes by consortium of Aspergillus niger MSK-7 and Trichoderma viride MSK-10

Abstract

The present work describes the production of cellulolytic enzymes (CMC-ase, FP-ase, -glucosidase) and
hemicellulolytic enzyme (xylanase) along with total extracellular protein by Aspergillus niger and
Trichoderma viride using submerged fermentation. Among seven different kinds of experiments,
secretion rate of protein and enzymes was investigated by mono- and co-cultures of A. niger and T.
viride using wheat bran as substrate with the supply of Eggins and Pugh salt medium. Co-culture of A.
niger and T. viride (when both were mixed together simultaneously) gave 30-50% higher production of
total protein (0.58 mg/ml) and enzymes; CMC-ase (2.79 U/ml/min), FP-ase (1.75 U/ml/min) and xylanase
(189.7 U/ml/min) than mono-cultures and all other combinations. Biosynthesis of -glucosidase was
found higher i.e., 4.66 U/ml/min in co-culture of A. niger and T. viride (when T. viride was mixed with 24
hours old culture of A. niger). Simultaneous co-culture of A. niger and T. viride was further optimized
with fermentation rate, different carbon sources, incubation temperature and different pH of
fermentation media.