Small and large scale genomic DNA isolation protocol for chickpea (Cicer arietinum L.), suitable for molecular marker and transgenic analyses
Keywords:
Cicer arietinum L., phenolics, restriction enzyme digestion, PCR amplification, Southern hybridizationAbstract
Chickpea is an important food legume crop with high nutritional value. Lack of appropriate DNA isolation protocol is
a limiting factor for any molecular studies of this crop. The present report describes a rapid and efficient protocol
for small and large scale preparation of superior quality and quantity of DNA from four cultivars (JG62, WR315, C235
and ICCV89314) compared to that of earlier reports. The yield of DNA through both the methods was estimated to be
approximately 80 g per g of plant tissue. Both small and large scale preparations were essentially suitable for PCR
and Southern blot hybridization analyses, which are the key steps in crop improvement programme through marker
development and genetic engineering techniques.
