Clarithromycin resistance and 23S rRNA mutations in Helicobacter pylori isolates in Iran
Keywords:
Polymerase chain reaction, clarithromycin, resistance, 23S rRNA, polymerase chain reaction - restriction fragment length polymorphism.Abstract
To determine the 23S rRNA point mutations in clarithromycin resistance of Helicobacter pylori strains
isolated from southwest, Iran. This was a cross-sectional survey, which was done on 263 patients who
referred to endoscopy department of Shehrekord university of medical sciences. According to gram
stain, urease, catalase, oxidase and polymerase chain reaction (PCR) H. pylori identified. Standard
National Committee for Clinical Laboratory Standard (NCCLS) method used for assessment of
clarithromycin resistance. Specific primers and restriction enzymes BsaI and MboII by PCR-RFLP were
used for analysis of A2143G and A2142G mutations. So for the detection of A2142C, specific primers
and PCR method were used. 84 strains of H. pylori (31.94%) determined by PCR method. Of 19 (22.62%)
clarithromycin resistant strains 13 (68.40%), 3 (15.78%), 2 (10.52%) had A2143G, A2142G, A2142C
respectively and one unknown mutation in 23S rRNA gene. Because of considerable resistance to
clarithromycin, direct diagnosis of this mutation by molecular approach in other parts of the country is
necessary
