Feasibility of using dot blot hybridization to detect Salmonella InvA, SpiC and SipC directly from clinical specimens
Keywords:
Salmonella, dot-blot hybridization, spiC, sipC, invA, clinical-samples.Abstract
Pathogenesis of Salmonella depends upon a large number of factors controlled by an array of genes that
synergise into actual virulence. The goal of this study was to detect Salmonella invA, spiC and sipC directly from
clinical specimens, using the dot blot hybridization assay. We detected invA , spiC and sipC as a one
combination from 4.5% (95% CI: 2.21 to 8.64) human feacal and 35.2% (95% CI: 26.4 to 45.0) poultry samples after
enrichment. Furthermore the dot blot method had a higher sensitivity than routine culture, before and after
enrichment. These results indicate that dot blot hybridization may be used to directly detect Salmonella invA,
spiC and sipC in clinical samples.
