Biological activity of the n-butanolic extract of Stachys pilifera
Keywords:
Antioxidant, antitumor, 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenol, antimicrobial.Abstract
The n-butanolic extract of Stachys pilifera growing in Iran have been screened for antimicrobial, antioxidant and
antitumor activity. The antimicrobial capacity of this extract was evaluated against Gram-positive and Gramnegative bacteria, and fungi. The extract was showed mildly significant activity against these microorganisms.
The antioxidant activities were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging
assays. IC50 values in the DPPH assay 2.8 mg/mL. This plant showed the high antioxidant activities. DPPH assay
results showed good correlations with the total phenolic contents of the plants, measured by the FolinCiocalteau assay: (r2
= 0.871, p < 0.0001). The results of antitumor activity were showed that the extract exhibit
antitumor properties. It was showed enhanced inhibitory activity compared to control compounds. It has also
been proposed that concentration plays a vital role in increasing the degree of inhabitation.
