An assay for detecting foodborn Salmonella using Loop-mediated isothermal amplification

Abstract

Loop-mediated isothermal amplification (LAMP) was a novel nucleic acid amplification method that amplified DNA
with high specificity, efficiency and rapidity under isothermal conditions using a set of four specially designed
primers and a DNA polymerase with strand displacement activity. In our research, LAMP was developed to detect
foodborn Salmonella in raw milk, a set of six primers, two outer, two inner and two loop primers, were designed from
Salmonella genomic DNA targeting invA. Temperature condition for detection of Salmonella was optimized to be 61°C.
At the same time, LAMP was found to be effective when the template was pure; however, the usefulness of LAMP
methods can be limited by the presence of inhibitors in the analysis of raw milk, unlike all the reports about LAMP, the
sensitivity of LAMP was lower than that of PCR methods.