Molecular diversity analysis of planctomycete like bacteria in inosine fermentation and municipal wastewater treatment systems

Abstract

In this study, the diversity of planctomycete-like bacteria in an inosine fermentation and a municipal
wastewater treatment plants was investigated by denaturing gradient gel electrophoresis (DGGE) of nested
polymerase chain reaction (nested PCR) amplified 16S rRNA gene fragments using planctomycete-specific
forward primer and a bacteria universal reverse primer. The bacterial community structures in the two
wastewater treatment systems were obviously different due to the different treatment processes and different
wastewater characteristics. The highest similarity of the microbial community structure happened in the two
samples from the acidification and the anaerobic tank in inosine fermentation plant reaching 67%. While the
two samples from the anaerobic and the aerobic tank of municipal wastewater treatment plant reached 66%.
Ten sequences were recovered from the DGGE gel and were assigned to Clostridia, Proteobacteria,
Bacteroidete and Candidate division based on the phylogenetic analysis. The two sequences grouped into
Candidate division had more than 92% similarities with the Planctomycete-like bacterial clones. However, no
anaerobic ammonium oxidation (anammox) activities were detected in the samples of these two wastewater
treatment plants.