Development of a sensitive spectrophotometeric method for determination of copper

Abstract

Simple and rapid spectrophotometeric procedure have been established for quantitation of Cu (II).This method is
based on the reaction between alizarin red S and boric acid in the solution of pH 8.5 to produce a red complex which
place between organic and aqueous phases. In presence of Cu (II), the complex of alizarin red S and boric acid is
dissociated and new complex between alizarin red S and metal ions formed which can be measured at the optimum
wavelength. All variables were studied to optimize the reaction conditions. Regression analysis of beer’s low plot
showed good correlation in concentration range 0.6 - 15 μg.mL-1. The molar absorptivity and detection limit are
calculated. The interference effects of the foreign cation have been examined.