Rapid high performance liquid chromatographic determination of chlorpropamide in human plasma

Abstract

Samples were extracted with dichloromethane and the organic layer evaporated to dryness. The residue
was dissolved in methanol, and 25 µl aliquot injected onto the column. Tolbutamide was used as the
internal standard for chlorpropamide. The UV detector response was linear over the range 0 – 300
µg/ml, with a correlation coefficient of 0.999 and detection limit of 1.30 ng/ml. Within day and betweenday assay variations was generally < 2.50%. No interference from endogenous constituent was
observed. The utility of the method was demonstrated by determine chlorpropamide in samples from
human volunteers following a single oral dose of 250 mg in drug interaction studies. The procedure is
simple and fast, requiring small volumes of plasma.
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