Evaluation of genetic variation within different population of Puntius filamentosus from southern Western Ghats

Abstract

The genetic variation in different population of the freshwater cyprinid Puntius filamentosus was
studied using restriction fragment length polymorphism (RFLP) analysis. Samples were collected from
five different locations of southern Western Ghats, India. The morphometric characters of population
from Alancholai showed little variation when compared with other population. The genomic size of the
different population of P. filamentosus found between 3.45 and 3.80 ng/mg. The result of RFLP analysis
showed that the population from Alancholai had distinct fragment length and scored high band volume
(12.430 nmoles). The result of cluster analysis showed that Alancholai population had distinct genetic
structure and it did not cluster with other population. The study inferred that the population from
Alancholai appeared to be unique among the other population of P. filamentosus.