Metabolic control of lignocellulose degradation by lignocellulose-degradation microbial community MC1

Abstract

This study was to reveal the limiting factors of lignocellulose degradation and further improve the
potential degradation of microbial community MC1, by pH manual adjustment (pHMA) and culture
medium partial replacement (CMPR) during the process of rice straw degradation. The results indicated
degradation rate in control (5% rice straw) increased, ranging from 33.3 to 67.5% via pHMA method, and
for treatment with CMPR which was elevated to 83.3%. Meanwhile, pH in control dropped to 5.4 and
remained after 20 days of incubation, while pH increased to 6.9 in sample with pHMA and 6.5 in sample
with CMPR. With molecular footprint technology, 9 bands undetected in previous work were discovered
in MC1 from the DGGE profiles. The results of soluble products with two treatments demonstrated that
CMPR technology could not only facilitate the degradation but also accumulated quantities of soluble
products compared to pHMA method which decreased quantities of soluble products.